bsm-61708R [Primary Antibody]
MELK Recombinant Antibody
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Host: Rabbit

Target Protein: MELK

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9833

Swiss Prot: Q14680

Source: KLH conjugated synthetic peptide derived from human MELK

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis.

Size: 100uL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 75


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with MELK monoclonal antibody, unconjugated (bsm-61708R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.