bsm-61711R [Primary Antibody]
alpha Tubulin Recombinant Antibody
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Host: Rabbit

Target Protein: alpha Tubulin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 854889

Swiss Prot: P09733

Source: KLH conjugated synthetic peptide derived from alpha Tubulin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 50


Cross Reactive Species: Yeast

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with alpha Tubulin monoclonal antibody, unconjugated (bsm-61711R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.