bsm-61714R [Primary Antibody]
GRK3 Recombinant Antibody
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Host: Rabbit

Target Protein: GRK3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 157

Swiss Prot: P35626

Source: KLH conjugated synthetic peptide derived from human GRK3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

TIMP3 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP1, MMP2, MMP3, MMP7, MMP9, MMP13, MMP14 and MMP15.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))

Predicted Molecular Weight: 80


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with GRK3 monoclonal antibody, unconjugated (bsm-61714R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRK3 Monoclonal Antibody, Unconjugated (bsm-61714R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRK3 Monoclonal Antibody, Unconjugated (bsm-61714R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRK3 Monoclonal Antibody, Unconjugated (bsm-61714R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.