bsm-61720R [Primary Antibody]
IKK epsilon Recombinant Antibody
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Host: Rabbit

Target Protein: IKK epsilon

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9641

Swiss Prot: Q14164

Source: KLH conjugated synthetic peptide derived from human IKK epsilon

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 80


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with IKK epsilon monoclonal antibody, unconjugated (bsm-61720R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK epsilon Monoclonal Antibody, Unconjugated (bsm-61720R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK epsilon Monoclonal Antibody, Unconjugated (bsm-61720R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.