bsm-61761R [Primary Antibody]
Prostate Secretory Protein/PSP Recombinant Antibody
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Host: Rabbit

Target Protein: Prostate Secretory Protein/PSP

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4477

Swiss Prot: P08118

Source: KLH conjugated synthetic peptide derived from human beta 2 Microseminoprotein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 13


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein/PSP monoclonal antibody, unconjugated (bsm-61761R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.