bsm-61802R [Primary Antibody]
Cadherin 16 Recombinant Antibody
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Host: Rabbit

Target Protein: Cadherin 16

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1014

Swiss Prot: O75309

Source: KLH conjugated synthetic peptide derived from human Cadherin 16

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Size: 100µL

Concentration: 1 mg/ml

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
IP(IP(1:20-50))

Predicted Molecular Weight: 90


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated(bsm-61802R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cadherin 16 Monoclonal Antibody, Unconjugated(bsm-61802R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Cadherin 16 monoclonal antibody, unconjugated (bsm-61802R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.