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Host: Rabbit
Target Protein: Nkx2.2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4821
Swiss Prot: O95096
Source: KLH conjugated synthetic peptide derived from human Nkx 2.2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Applications:
WB(WB(1:500-2000))
IF(ICC)(IF(ICC)(1:50-200))
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Nkx2.2 monoclonal antibody, unconjugated (bsm-61814R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed A673 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Nkx2.2) monoclonal Antibody, unconjugated (bsm-61814R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.