bsm-61838R [Primary Antibody]
SMURF2 Recombinant Antibody
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Host: Rabbit

Target Protein: SMURF2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 64750

Swiss Prot: Q9HAU4

Source: KLH conjugated synthetic peptide derived from human SMURF2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling.

Size: 100uL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 86


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with SMURF2 monoclonal antibody, unconjugated (bsm-61838R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.