bsm-61880R [Primary Antibody]
Sprouty 4 Recombinant Antibody
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Host: Rabbit

Target Protein: Sprouty 4

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 81848

Swiss Prot: Q9C004

Source: KLH conjugated synthetic peptide derived from human Sprouty 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras.Probably impairs the formation of GTP-Ras.Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
FCM(FCM(1:50-100))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))

Predicted Molecular Weight: 33


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Sprouty 4 monoclonal antibody, unconjugated (bsm-61880R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.