bsm-61905R-BF750

DATASHEET

Host: Rabbit

Target Protein: HIF1AN

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 55662

Swiss Prot: Q9NWT6

Source: KLH conjugated synthetic peptide derived from human HIF 1 alpha inhibitor

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins.

Conjugation: AbBy Fluor-750

Excitation/ Emission: 749nm/775nm

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:500-2000))
FCM(FCM(1ug/test))
IF(IHC-P)(IF(IHC-P)(1:200-500))
IF(ICC)(IF(ICC)(1:100-200))

Predicted Molecular Weight: 40

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.