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Host: Rabbit
Target Protein: NOXA2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4688
Swiss Prot: P19878
Source: KLH conjugated synthetic peptide derived from human NOXA2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100uL
Concentration: Lot dependent
Applications:
WB(1:500-2000)
IHC-P(1:20-50)
IHC-F(1:100-500)
IP(1-2ug)
IF
ICC/IF(1:50-100)
Predicted Molecular Weight: 60
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
THP-1 (H) cells were treated with or without PMA (100nM) for 48 h, 25 μg total protein per lane of cell lysates (see on figure) probed with NOXA2 monoclonal antibody, unconjugated (bsm-61913R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (bsm-61913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (bsm-61913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (bsm-61913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NOXA2 Monoclonal Antibody, Unconjugated (bsm-61913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.