bsm-61946R [Primary Antibody]
GNL3 Recombinant Antibody
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Host: Rabbit

Target Protein: GNL3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 26354

Swiss Prot: Q9BVP2

Source: KLH conjugated synthetic peptide derived from human GNL3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 62


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with GNL3 monoclonal antibody, unconjugated (bsm-61946R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GNL3) monoclonal Antibody, unconjugated (bsm-61946R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.