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Host: Rabbit
Target Protein: CHD1L
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 9557
Swiss Prot: Q86WJ1
Source: KLH conjugated synthetic peptide derived from human CHD1L
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Applications:
WB(WB(1:500-2000))
FCM(FCM(1:50-100))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))
Predicted Molecular Weight: 101
Cross Reactive Species: Human
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CHD1L antibody (bsm-61977R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with CHD1L monoclonal antibody, unconjugated (bsm-61977R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.