bsm-62007R [Primary Antibody]
ATP1B3 Recombinant Antibody
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Host: Rabbit

Target Protein: ATP1B3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 483

Swiss Prot: P54709

Source: KLH conjugated synthetic peptide derived from human ATP1B3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ATP1B3 monoclonal antibody, unconjugated (bsm-62007R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATP1B3 Monoclonal Antibody, Unconjugated (bsm-62007R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.