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Host: Rabbit
Target Protein: HIPK2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 28996
Swiss Prot: Q9H2X6
Source: KLH conjugated synthetic peptide derived from human HIPK2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100uL
Concentration: Lot dependent
Applications:
WB(WB(1:1000-2000))
FCM(FCM(1μg/Test))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))
Predicted Molecular Weight: 131
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIPK2) monoclonal Antibody, unconjugated (bsm-62009R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HIPK2 antibody (bsm-62009R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.