bsm-62087R [Primary Antibody]
NUDC Recombinant Antibody
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Host: Rabbit

Target Protein: NUDC

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10726

Swiss Prot: Q9Y266

Source: KLH conjugated synthetic peptide derived from human NUDC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a role in neurogenesis and neuronal migration.Necessary for correct formation of mitotic spindles and chromosome separation during mitosis.Necessary for cytokinesis and cell proliferation.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 38


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NUDC monoclonal antibody, unconjugated (bsm-62087R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (bsm-62087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (bsm-62087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (bsm-62087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (bsm-62087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.