bsm-62123R-Biotin [Conjugated Primary Antibody]
GluR1 Recombinant Antibody, Biotin Conjugated
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Host: Rabbit

Target Protein: GluR1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2890

Swiss Prot: P42261

Source: KLH conjugated synthetic peptide derived from human GluR1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

AMPA- (ŚÁ-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100uL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IP(IP(1:20-50))

Predicted Molecular Weight: 102


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum (hippocampus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GluR1 Monoclonal Antibody (bsm-62123R-Bio) at 1:500 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum (hippocampus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GluR1 Monoclonal Antibody (bsm-62123R-Bio) at 1:500 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GluR1 Monoclonal Antibody (bsm-62123R-Bio) at 1:500 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GluR1 Monoclonal Antibody (bsm-62123R-Bio) at 1:500 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.