bsm-62130R [Primary Antibody]
G3BP1 Recombinant Antibody
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Host: Rabbit

Target Protein: G3BP1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10146

Swiss Prot: Q13283

Source: KLH conjugated synthetic peptide derived from human G3BP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Protein involved in various processes, such as stress granule formation and innate immunity.Plays an essential role in stress granule formation.Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress.Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1ug/Test)
IHC-F(1:100-500)
IP(1-2ug)
IF()
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with G3BP1 monoclonal antibody, unconjugated (bsm-62130R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with G3BP1 Monoclonal Antibody, Unconjugated (bsm-62130R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.