bsm-62150R [Primary Antibody]
Legumain Recombinant Antibody
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Host: Rabbit

Target Protein: Legumain

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5641

Swiss Prot: Q99538

Source: A synthesized peptide derived from human Legumain

Purification: purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Has a strict specificity for hydrolysis of asparaginyl bonds.Can also cleave aspartyl bonds slowly, especially under acidic conditions.Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)

Predicted Molecular Weight: 49 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Legumain monoclonal antibody, unconjugated (bsm-62150R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.