bsm-62168R [Primary Antibody]
PAF1 Recombinant Antibody
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Host: Rabbit

Target Protein: PAF1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 54623

Swiss Prot: Q8N7H5

Source: KLH conjugated synthetic peptide derived from human PAF1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with PAF1 monoclonal antibody, unconjugated (bsm-62168R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.