bsm-62183R [Primary Antibody]
ZNF348/Kaiso Recombinant Antibody
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Host: Rabbit

Target Protein: ZNF348/Kaiso

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10009

Swiss Prot: Q86T24

Source: KLH conjugated synthetic peptide derived from human KAISO

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters.

Size: 100uL

Concentration: Lot dependent

Applications: WB(1:300-5000)

Predicted Molecular Weight: 74


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ZNF348/Kaiso monoclonal antibody, unconjugated (bsm-62183R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.