bsm-62263R [Primary Antibody]
MCM4 Recombinant Antibody
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Host: Rabbit

Target Protein: MCM4

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4173

Swiss Prot: P33991

Source: KLH conjugated synthetic peptide derived from human MCM4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 97


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MCM4) monoclonal Antibody, unconjugated (bsm-62263R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


25 ug total protein per lane of various lysates (see on figure) probed with MCM4 monoclonal antibody, unconjugated (bsm-62263R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.