bsm-62331R [Primary Antibody]
CXCL5 Recombinant Antibody
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Host: Rabbit

Target Protein: CXCL5

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6374

Swiss Prot: P42830

Source: KLH conjugated synthetic peptide derived from human CXCL5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))

Predicted Molecular Weight: 12


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A549 (H) cells were treated with or without IL-1 for 24 h, PMA (200 nM) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with CXCL5 monoclonal antibody, unconjugated (bsm-62331R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed A549(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CXCL5) monoclonal Antibody, unconjugated (bsm-62331R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.