bsm-62344R [Primary Antibody]
MonoMethyl-Histone H2B type 2E (Lys117) Recombinant Antibody
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Host: Rabbit

Target Protein: Histone H2B type 2E

Modification Site: Lys117

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 8349

Swiss Prot: Q16778

Source: KLH conjugated synthetic peptide derived from human Histone H2B type 2E around the methylation site of K117

Purification: Purified Polyclonal A

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
ICC/IF(1:50-100)

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MonoMethyl-Histone H2B type 2E (Lys117) Monoclonal Antibody, Unconjugated (bsm-62344R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.