bsm-62393R [Primary Antibody]
ERCC8 Recombinant Antibody
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Host: Rabbit

Target Protein: ERCC8

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1161

Swiss Prot: Q13216

Source: KLH conjugated synthetic peptide derived from human ERCC8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 44


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ERCC8 monoclonal antibody, unconjugated (bsm-62393R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.