bsm-62399R [Primary Antibody]
NLK Recombinant Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NLK

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 51701

Swiss Prot: Q9UBE8

Source: KLH conjugated synthetic peptide derived from human NLK

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination.Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2.Negative regulator of the canonical Wnt/beta-catenin signaling pathway.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 58


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NLK monoclonal antibody, unconjugated (bsm-62399R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.