bsm-62423R [Primary Antibody]
p66 alpha/GATAD2A Recombinant Antibody
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Host: Rabbit

Target Protein: p66 alpha/GATAD2A

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 54815

Swiss Prot: Q86YP4

Source: KLH conjugated synthetic peptide derived from human p66 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.Enhances MBD2-mediated repression.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1-2ug)
Flow-Cyt(1:50-200)
ICC/IF(1:50-100)

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with p66 alpha/GATAD2A monoclonal antibody, unconjugated (bsm-62423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with p66 alpha/GATAD2A Monoclonal Antibody, Unconjugated (bsm-62423R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.