DATASHEET
Host:
Rabbit
Target Protein:
GBP1
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
2633
Swiss Prot:
P32455
Source:
KLH conjugated synthetic peptide derived from human GBP1
Purification:
Purified Polyclonal A
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mediates the exchange of one Ca2+ ion against three to four Na+ ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca2+ levels and Ca2+-dependent cellular processes.
VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with GBP1 monoclonal antibody, unconjugated (bsm-62454R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GBP1 Monoclonal Antibody, Unconjugated (bsm-62454R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.