bsm-62454R [Primary Antibody]
GBP1 Recombinant Antibody
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Host: Rabbit

Target Protein: GBP1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2633

Swiss Prot: P32455

Source: KLH conjugated synthetic peptide derived from human GBP1

Purification: Purified Polyclonal A

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mediates the exchange of one Ca2+ ion against three to four Na+ ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca2+ levels and Ca2+-dependent cellular processes.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
ICC/IF(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with GBP1 monoclonal antibody, unconjugated (bsm-62454R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GBP1 Monoclonal Antibody, Unconjugated (bsm-62454R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.