bsm-62459R [Primary Antibody]
MED4 Recombinant Antibody
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Host: Rabbit

Target Protein: MED4

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 29079

Swiss Prot: Q9NPJ6

Source: KLH conjugated synthetic peptide derived from human MED4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 30


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with MED4 monoclonal antibody, unconjugated (bsm-62459R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.