bsm-62549R [Primary Antibody]
TRPV5 Recombinant Antibody
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Host: Rabbit

Target Protein: TRPV5

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 56302

Swiss Prot: Q9NQA5

Source: KLH conjugated synthetic peptide derived from human TRPV5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine.Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules.The channel is activated by low internal calcium level and the current exhibits an inward rectification.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))

Predicted Molecular Weight: 83


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with TRPV5 monoclonal antibody, unconjugated (bsm-62549R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TRPV5 Monoclonal Antibody, Unconjugated (bsm-62549R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TRPV5 Monoclonal Antibody, Unconjugated (bsm-62549R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TRPV5 Monoclonal Antibody, Unconjugated (bsm-62549R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TRPV5 Monoclonal Antibody, Unconjugated (bsm-62549R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TRPV5 Monoclonal Antibody, Unconjugated (bsm-62549R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.