bsm-62551R [Primary Antibody]
KLK8 Recombinant Antibody
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Host: Rabbit

Target Protein: KLK8

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 11202

Swiss Prot: O60259

Source: KLH conjugated synthetic peptide derived from human Kallikrein 8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 28


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with KLK8 monoclonal antibody, unconjugated (bsm-62551R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with KLK8 Monoclonal Antibody, Unconjugated (bsm-62551R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.