bsm-62586R [Primary Antibody]
HNF4A Recombinant Antibody
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Host: Rabbit

Target Protein: HNF4A

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 15378

Swiss Prot: P41235

Source: KLH conjugated synthetic peptide derived from human HNF 4 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 53


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HNF4A) monoclonal Antibody, unconjugated (bsm-62586R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


25 ug total protein per lane of various lysates (see on figure) probed with HNF4A monoclonal antibody, unconjugated (bsm-62586R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.