bsm-62623R [Primary Antibody]
UBA3 Recombinant Antibody
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Host: Rabbit

Target Protein: UBA3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9039

Swiss Prot: Q8TBC4

Source: KLH conjugated synthetic peptide derived from human UBA3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with UBA3 monoclonal antibody, unconjugated (bsm-62623R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.