bsm-62672R [Primary Antibody]
WDR1 Recombinant Antibody
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Host: Rabbit

Target Protein: WDR1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9948

Swiss Prot: O75083

Source: KLH conjugated synthetic peptide derived from human WDR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing.Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions.Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
ICC/IF(1:50-100)

Predicted Molecular Weight: 66


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with WDR1 monoclonal antibody, unconjugated (bsm-62672R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with WDR1 Monoclonal Antibody, Unconjugated (bsm-62672R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with WDR1 Monoclonal Antibody, Unconjugated (bsm-62672R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.