bsm-62715R [Primary Antibody]
AER61 Recombinant Antibody
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Host: Rabbit

Target Protein: AER61

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 285203

Swiss Prot: Q5NDL2

Source: KLH conjugated synthetic peptide derived from human EOGT

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)

Predicted Molecular Weight: 62


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with AER61 monoclonal antibody, unconjugated (bsm-62715R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with AER61 monoclonal antibody, unconjugated (bsm-62715R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.