bsm-62739R [Primary Antibody]
NOLC1 Recombinant Antibody
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Host: Rabbit

Target Protein: NOLC1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9221

Swiss Prot: Q14978

Source: KLH conjugated synthetic peptide derived from human NOLC1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification.Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.

Size: 100uL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:50-200)
IHC-F(1:50-200)
IF(1:50-200)
ICC/IF(1:50-100)

Predicted Molecular Weight: 74


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of HeLa cell lysate. Using NOLC1 (bsm-62739R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NOLC1) monoclonal Antibody, unconjugated (bsm-62739R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.