bsm-62748R [Primary Antibody]
NDUFS2 Recombinant Antibody
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Host: Rabbit

Target Protein: NDUFS2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4720

Swiss Prot: O75306

Source: KLH conjugated synthetic peptide derived from human NDUFS2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.

Size: 100uL

Concentration: 1 mg/ml

Applications: WB(1:1000-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IP(1:50-100)
IF(1:100-500)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NDUFS2 monoclonal antibody, unconjugated (bsm-62748R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Stoach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Stoach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Stoach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFS2 Monoclonal Antibody, Unconjugated (bsm-62748R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.