bsm-62749R [Primary Antibody]
NDUFB4 Recombinant Antibody
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Host: Rabbit

Target Protein: NDUFB4

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4710

Swiss Prot: O95168

Source: KLH conjugated synthetic peptide derived from human NDUFB4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
IHC-P(1ug/Test)
IHC-F(1:50-100)
IF
ICC/IF(1:50-100)

Predicted Molecular Weight: 15


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NDUFB4 monoclonal antibody, unconjugated (bsm-62749R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with NDUFB4 monoclonal antibody, unconjugated (bsm-62749R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (bsm-62749R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (bsm-62749R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (bsm-62749R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.