bsm-62777R [Primary Antibody]
NDUFAB1 Recombinant Antibody
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Host: Rabbit

Target Protein: NDUFAB1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4706

Swiss Prot: O14561

Source: KLH conjugated synthetic peptide derived from human NDUFAB1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Carrier of the growing fatty acid chain in fatty acid biosynthesis.Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (PubMed:27626371).

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IP(1:50-200)
IF
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 17


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NDUFAB1 monoclonal antibody, unconjugated (bsm-62777R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (bsm-62777R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (bsm-62777R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (bsm-62777R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (bsm-62777R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFAB1 Monoclonal Antibody, Unconjugated (bsm-62777R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.