bsm-62800R [Primary Antibody]
p45 NF-E2 Recombinant Antibody
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Host: Rabbit

Target Protein: p45 NF-E2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4778

Swiss Prot: Q16621

Source: KLH conjugated synthetic peptide derived from human NFE2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:50-100)
IHC-F(1:100-500)
IP(1-2ug)
IF
ICC/IF(1:50-100)

Predicted Molecular Weight: 41


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with p45 NF-E2 monoclonal antibody, unconjugated (bsm-62800R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.