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Host: Rabbit
Target Protein: ITCH/AIP4
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 83737
Swiss Prot: Q96J02
Source: KLH conjugated synthetic peptide derived from human ITCH
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Applications:
FCM(FCM(1:50-100))
IF(ICC)(IF(ICC)(1:100-500))
Predicted Molecular Weight: 103
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (ITCH/AIP4) monoclonal Antibody, unconjugated (bsm-62844R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ITCH/AIP4 antibody (bs-62844R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.