bsm-62859R [Primary Antibody]
KDM1/LSD1 Recombinant Antibody
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Host: Rabbit

Target Protein: KDM1/LSD1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 23028

Swiss Prot: O60341

Source: KLH conjugated synthetic peptide derived from human KDM1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 93


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (KDM1/LSD1) monoclonal Antibody, unconjugated (bsm-62859R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.