bsm-62860R [Primary Antibody]
Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody
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Host: Rabbit

Target Protein: PKA C alpha/beta/gamma

Modification Site: Thr197

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5568

Swiss Prot: P22612,P17612,P22694

Source: KLH conjugated synthetic peptide derived from human PKA C alpha around the phosphorylation site of T197

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.

Size: 100uL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:50-200))
IHC-F(IHC-F(1:50-200))
IF(IHC-P)(IF(IHC-P)(1:50-200))

Predicted Molecular Weight: 40,41


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C alpha/beta/gamma (T197) monoclonal antibody, unconjugated (bsm-62860R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.