bsm-62874R [Primary Antibody]
Osteocalcin Recombinant Antibody
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Host: Rabbit

Target Protein: Osteocalcin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 632

Swiss Prot: P02818

Source: A synthesized peptide derived from human Osteocalcin

Purification: purified by Protein A.

Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation. Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
IF(1:100-500)
IHC-P(1:100-500)

Predicted Molecular Weight: 11 kDa


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li Lan. et al. Mechanically induced M2 macrophages are involved in bone remodeling of the midpalatal suture during palatal expansion. PROG ORTHOD. 2024 Dec;25(1):1-12Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human osteosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Monoclonal Antibody, Unconjugated (bsm-62874R) at 1:300 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.