bsm-62878R [Primary Antibody]
NELFB Recombinant Antibody
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Host: Rabbit

Target Protein: NELFB

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 25920

Swiss Prot: Q8WX92

Source: KLH conjugated synthetic peptide derived from human NELF B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II.The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:50-200)
IHC-F(1:50-200)
IF(1:50-200)
ICC/IF(1:50-200)

Predicted Molecular Weight: 66


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with NELFB monoclonal antibody, unconjugated (bsm-62878R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.