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Host: Rabbit
Target Protein: Lamin A/C
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4000
Swiss Prot: P02545
Source: KLH conjugated synthetic peptide derived from human Lamin A/C
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Conjugation: AbBy Fluor-488
Excitation/ Emission: 499nm/519nm
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:1000-5000))
FCM(FCM(1:50-200))
IF(IHC-P)(IF(IHC-P)(1:200-800))
IF(ICC)(IF(ICC)(1:100-200))
Predicted Molecular Weight: 74
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Lamin A/C) monoclonal Antibody,FITC conjugated (bsm-62886R-BF488) 1:100, 90 min at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-Lamin A/C antibody (bsm-62886R-BF488,1:100);Isotype Control (orange): Rabbit IgG/BF488 (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.