DATASHEET
Host:
Rabbit
Target Protein:
MYOD1
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
4654
Swiss Prot:
P15172
Source:
KLH conjugated synthetic peptide derived from human MYOD1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with MYOD1 monoclonal antibody, unconjugated (bsm-62915R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with MYOD1 monoclonal antibody, unconjugated (bsm-62915R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.