bsm-62970R [Primary Antibody]
Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody
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Host: Rabbit

Target Protein: RPS6KB1

Modification Site: Thr421/Ser424

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6198

Swiss Prot: P23443

Source: KLH conjugated synthetic peptide derived from human p70 S6 Kinase around the phosphorylation site of T421/S424

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IP(IP(1:20-50))

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-RPS6KB1 monoclonal antibody, unconjugated (bsm-62970R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.