bsm-62975R [Primary Antibody]
EDG3 Recombinant Antibody
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Host: Rabbit

Target Protein: EDG3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1903

Swiss Prot: Q99500

Source: KLH conjugated synthetic peptide derived from human EDG3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:1000-2000))
IHC-P(IHC-P(1:100-200))
IHC-F(IHC-F(1:100-200))
IF(IHC-P)(IF(IHC-P)(1:100-200))
IF(ICC)(IF(ICC)(1:50-200))

Predicted Molecular Weight: 42


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(bsm-62975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(bsm-62975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(bsm-62975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(bsm-62975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EDG3 Monoclonal Antibody, Unconjugated(bsm-62975R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.